![]() ![]() HiTrap IXSelect: pour la purification du facteur de coagulation IX. HiTrap IgY: designed for purification of IgY. HiTrap IgM: designed for purification of monoclonal IgM. HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures. HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants. HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines. Purification of immunoglobulins Acronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules.It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as required Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer.HiTrap columns are available in 1 ml and 5 ml bed volume.Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values. ![]() Wash with at least 2 CV ultra pure water.ĥ. Read these instructions carefully before using HiTrap columns. The columns are used in an optimal way with liquid chromatography systems such as ÄKTA systems, but can also be operated with a syringe or a peristaltic pump. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.Ĥ. HiTrap Capto Q, Capto S and Capto DEAE columns provide fast, reproducible and easy separations in a convenient format. High resolution columnsfor laboratory scale ion exchange chromatography. They are well-suited for the preparation of highly pure proteins for structural studies and for protein analysis. Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.ġ. Capto HiRes Q and Capto HiRes S are prepacked columns for high-resolution purification of proteins and other biomolecules by ion exchange chromatography. This prevents clogging and increases the life time of the column when loading large sample volumes. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. You would need a negatively charged column like CM. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.Ģ. Therefore it would not likely stick to a Q column which is made of quaternary positively charged amines. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. These columns are easily connected in series to increase bed height to 20 cm.įast results and minimal consumption of sample and buffer consumption through the small bed volume.īioProcess columns packed with the same chromatography resin and using the same linear fluid velocity can produce scalable, reproducible results.ġ. ![]() Prepacked with Capto Q strong anion exchange chromatography resin.Ĭonvenient method optimization and parameter screening because of the 10cm bed height. However, Capto adhere can also be used in bind/elute mode and for applications other than MAbs. It was originally designed for post-protein A purification of MAbs at process scale in flowthrough mode. HiScreen Capto Q is a column packed with a strong anion exchange modern resin, combining speed and capacity. Capto adhere is a multimodal strong anion exchanger for BioProcess applications. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |